【个人简介】

朱作斌，博士，副教授，硕士生导师。遗传学科负责人，徐州医科大学生命科学学院遗传学创新平台负责人，全国大学生生命科学创新创业优秀指导老师，江苏省多基因重大疾病精准诊疗工程研究中心副主任，徐州医科大学遗传基础与临床应用重点实验室副主任，以色列科学基金会评审专家、iMeta青年编委、Frontiers in Pharmacology、 Frontiers in microbiology 期刊客座编辑，中国人体健康科技促进会临床微生物与感染精准检验专业委员会委员、江苏省妇幼保健协会男性生殖分会委员、江苏省遗传学会微生物遗传委员会委员，江苏省免疫学会免疫遗传学组委员。

研究方向：生殖衰老的遗传机制；微生物与宿主互作。本课题组依托徐州市医学遗传与转化工程中心及徐州医科大学遗传基础与临床应用重点实验室，以秀丽隐杆线虫、果蝇和小鼠为动物模型进行生殖、衰老及相关疾病的遗传机制研究的研发。

主持国家自然科学基金、江苏省自然科学基金、徐州市基础研发计划等9课题9项，获批总经费300余万元，参与国家重点基础研究发展计划项目1项，国家自然科学基金面上项目2项。以第一作者或通讯作者发表SCI论文40余篇，其中IF>10分5篇，IF>5分论文12篇。获得中国中西医结合科技奖二等奖、江苏省医学科技奖三等奖和淮海科学技术奖二等奖各1项，申请国家发明专利10项，软件著作权3项。    

讲授《医学遗传学》、《系统生物学》、《高级医学遗传学》等课程。主编教材《系统生物学》、共同主编英文专著《Recent Advances in Bacterial Biofilm Studies》，参编《医学遗传学》教材一部。指导大学生获得江苏省大学生创新计划3项，指导研究生获得江苏省研究生创新课题3项。

【主持课题】

[1]      江苏省自然科学基金面上项目：cf-mtDNA调控生殖衰老的机制及酵母天然产物干预研究（BK20241953），15万元，2024.09-2027.08，主持；

[2]      徐州市应用基础研究项目，tRNA衍生片段致精子发生障碍的机制及特异性小分子药物干预作用研究（KC23009），10万元，2023.09-2025.08，主持（在研）

[3]      横向研究课题（徐州市中心医院），精子发生遗传标志物研究，2022.01-2024.12，40万元，主持；

[4]      横向研究课题（江苏阔然生物医药有限公司），基于尿液的膀胱癌甲基化无创诊断研究，100万元，2023.01-2025.12，共同主持；

[5]      徐州市重大研发计划（社会发展）项目，基于淮海地区遗传和环境因素系统分析的精子发生发育障碍临床精准诊疗研究（KC22096），120万，2022.09-2024.08年，共同主持（在研）；

[6]      江苏省自然科学基金青年项目，衰老基因调控网络的探究及相关基因筛选验证（BK20170250），20万元，2017.09-2020.08，主持（已结题）；

[7]      国家自然科学基金，核基因组-线粒体基因组相互作用基因的筛选及与衰老关系的研究（81701390），20万元，2018.01-2020.12（已结题）；

[8]      徐州市基础研发计划青年人才项目，线粒体-核基因组相互作用与线虫生殖关系的研究（KC19057），9万元，2019.09-2021.08，主持（已结题）；

[9]  徐州医科大学优秀人才资金，核基因组与线粒体基因组相互作用与生殖衰老关系的研究（D2017003），30万元，2017.01-2020.12，主持（已结题）。

【发表文章】

[1]    Cong, L., Y. Zhou, Y. Zhang, S. Mao, C. Chen, L. Wang, X. Li, Z. Zhang,  Zhu ZB* and Y. Li* (2025). "BTS1-knockout Saccharomyces cerevisiae with broad-spectrum antimicrobial activity through lactic acid accumulation." Front Cell Infect Microbiol 15: 1494149.

[2]    Mao, S., Y. Zhang, C. Chen, L. Cong, Zhu ZB*, Z. Xie* and Y. Li* (2025). "Diagnosis Accuracy of Raman Spectroscopy in the Identification of Pathogenic Bacteria." Biotechnol Appl Biochem: e2741.

[3]    Sun, Q., Y. Li, W. Yang, W. Feng, J. Zhou, Y. Cao, B. Zhang, Zhu ZB* and C. Han* (2024). "CircMYH9/miR-133a-3p/CXCR4 axis: a novel regulatory network in sperm fertilization and embryo development." Mol Biomed 5(1): 69.

[4]    Wang, W., Y. Liu, Z. H. Zhou, K. Pang, J. K. Wang, P. F. Huan, J. R. Lu, T. Zhu,  Zhu ZB* and C. H. Han* (2025). "Effects of human umbilical cord-derived mesenchymal stem cell therapy for cavernous nerve injury-induced erectile dysfunction in the rat model." Asian J Androl.

[5]    Wang, W., Y. Liu,  Zhu ZB, K. Pang, J. K. Wang, J. Gu, Z. B. Li, J. Wang, Z. D. Shi and C. H. Han (2024). "Research Advances in Stem Cell Therapy for Erectile Dysfunction." BioDrugs 38(3): 353-367.

[6]    Xu, J., Y. Yang, G. Guan, Y. Gao, Q. Sun, G. Yuan, X. Zhang, J. Yang, W. Yang*,  Zhu ZB*and C. Han* (2024). "Prediction of Unexplained Recurrent Miscarriages Using Thromboelastography." Int J Womens Health 16: 1573-1581.

[7]    Zhao, Q., Z.  Zhu ZB, J. Li, L. Zhang and L. Wang (2024). "Editorial: Untangle the broad connections and tight interactions between human microbiota and complex diseases through data-driven approaches, volume II." Front Microbiol 15: 1449474.

[8]     Zhu ZB*., C. Han and S. Huang (2024). "New insights shed light on the enigma of genetic diversity and species complexity." Sci China Life Sci 67(12): 2774-2776.

[9]  Ying Li; Simin Xu; Liying Wang; Hao Shi; Han Wang; Ziyi Fang; Yufan Hu; Jiayu Jin; Yujie Du; Mengqiong Deng; Liang Wang; Zhu ZB*; Gut microbial genetic variation modulates host lifespan, sleep, and motor performance, ISME J, 2023, 17(10): 1733-1740

[10]Fen Li; Mengmeng Wang; Qinghua Liu; Zhang-Wen Ma; Junjiao Wang; Ziyi Wang; Jiawei Tang; Jingwen Lyu; Zhu ZB*; Liang Wang* ; Molecular mechanisms of glycogen particle assembly in Escherichia coli, Carbohydrate Polymers, 2023, 299: 1-9

[11]Chaoqun Chen,Zhiyu Xie,Liu Cong,Shanshan Mao,Liying Wang,Yalun Wu,Yu Zhang,Qing Zhou ,Wenqiang Chang, Zhu ZB*，Ying, Li*，Antifungal activity of a maleimide derivative: disruption of cell membranes and interference with iron ion homoeostasis. Mycology. doi.org/10.1080/21501203.2024.2330403

[12]Guixue G, Yifu P, Xiaofeng T, Qian S, Yuan G, Wen Y, Conghui H, Zhu ZB*. Investigating the causal impact of polycystic ovary syndrome on gestational diabetes mellitus: a two-sample Mendelian randomization study. Front Endocrinol (Lausanne). 2024 Feb 5;15:1337562. doi: 10.3389/fendo.2024.1337562. PMID: 38375192;

[13]Shi ZD^#, Sun Z^#, Zhu ZB^#, Liu X, Chen JZ, Hao L, Zhu JF, Pang K, Wu D, Dong Y, Liu YF, Chen WH, Liang Q, Zhuo SC, Han CH. Integrated single-cell and spatial transcriptomic profiling reveals higher intratumour heterogeneity and epithelial-fibroblast interactions in recurrent bladder cancer. Clin Transl Med. 2023 Jul;13(7):e1338. doi: 10.1002/ctm2.1338. PMID: 37488671; PMCID: PMC10366350.

[14]Cong L, Chen C, Mao S, Han Z, Zhu ZB*, Li Y*. Intestinal bacteria-a powerful weapon for fungal infections treatment. Front Cell Infect Microbiol. 2023 Jun 2;13:1187831. doi: 10.3389/fcimb.2023.1187831. PMID: 37333850; PMCID: PMC10272564.

[15]Wang L^#, Zhu ZB^#, Zhang L, Li J, Zhao Q. Editorial: Untangle the broad connections and tight interactions between human microbiota and complex diseases through data-driven approaches. Front Microbiol. 2023 Feb 15;14:1157579. doi: 10.3389/fmicb.2023.1157579. PMID: 36876103; PMCID: PMC9975743.

[16]Li Y, Chen C, Cong L, Mao S, Shan M, Han Z, Mao J, Xie Z, Zhu ZB*. Inhibitory effects of a maleimide compound on the virulence factors of Candida albicans. Virulence. 2023 Dec;14(1):2230009. doi: 10.1080/21505594.2023.2230009. PMID: 37367101; PMCID: PMC10321171.

[17]Chen C, Zhou Z, Cong L, Shan M, Zhu ZB*, Li Y*. Rapid identification of methicillin-resistant Staphylococcus aureus by MALDI-TOF MS: A meta-analysis. Biotechnol Appl Biochem. 2023 Jun;70(3):1217-1229. doi: 10.1002/bab.2433. Epub 2023 Jan 1. PMID: 36575908.

[18]Wang L, Zhang XD, Tang JW, Ma ZW, Usman M, Liu QH, Wu CY, Li F, Zhu ZB*, Gu B*. Machine learning analysis of SERS fingerprinting for the rapid determination of Mycobacterium tuberculosis infection and drug resistance. Comput Struct Biotechnol J. 2022 Sep 26;20:5364-5377. doi: 10.1016/j.csbj.2022.09.031.

[19]Fen Li, Meng-Meng Wang, Qing-Hua Liu, Zhang-Wen Ma, Jun-Jiao Wang , Yi Wang f, Jia-Wei Tang, Zhu ZB*, Liang Wang*. Molecular mechanisms of glycogen particle assembly in Escherichia coli. Carbohydrate Polymers. 2022，99 (2023) 120200

[20]Zhu Y, Li Y, Wang Y, Wang L, Shi P, Du X, Zhang Y, Song Y, Zhu ZB*. Mitochondrial DNA polymorphisms in COX1 affect the lifespan of Caenorhabditis elegans through nuclear gene dct-15. Gene. 2022 Dec 15;845:146776. doi: 10.1016/j.gene.2022.146776. Epub 2022 Sep 5.

[21]Han X, Hao L, Shi Z, Li Y, Wang L, Li Z, Zhang Q, Hu F, Cao Y, Pang K, Zhu ZB*. Seminal plasma extracellular vesicles tRF-Val-AAC-010 can serve as a predictive factor of successful microdissection testicular sperm extraction in patients with non-obstructive azoospermia. Reprod Biol Endocrinol. 2022 Jul 22;20(1):106. doi: 10.1186/s12958-022-00978-3.

[22]Zhu Y, Li Y, Zhang Q, Song YJ, Wang L, Zhu ZB*. Interactions between intestinal microbiota and neural mitochondria: a new perspective on communicating pathway from gut to brain. Frontiers in Microbiology. 2022; doi.10.3389/fmicb.2022.798917

[23]Zhang, Q, Liu Z, Han X, Li Y, Xia T, Zhu Y, Li ZB, Wang L, Hao L, Hu FF, Cao YJ, Han CH and Zhu ZB*. Circulatory exosomal tRF-Glu-CTC-005 and tRF-Gly-GCC-002 serve as predictive factors of successful microdissection testicular sperm extraction in patients with nonobstructive azoospermia. Fertility and Sterility. 2021 S0015-0282(21)02213-5

[24]Tang JW, Liu QH, Yin XC, Pan YC, Wen PB, Liu X, Kang XX, Gu B, Zhu ZB*, Wang L. Comparative Analysis of Machine Learning Algorithms on Surface Enhanced Raman Spectra of Clinical Staphylococcus Species. Front Microbiol. 2021 Aug 31;12:696921. doi: 10.3389/fmicb.2021.696921. PMID: 34531835; PMCID: PMC8439569.

[25]Zhu ZB, Wang L, Qian H, Gu F, Li Y, Zhang H, Chen Y, Shi J, Ma P, Bao C, Gu B. Comparative genome analysis of 12 Shigella sonnei strains: virulence, resistance, and their interactions. Int Microbiol. 2021 Jan;24(1):83-91. doi: 10.1007/s10123-020-00145-x. Epub 2020 Sep 3. PMID: 32880768.

[26]Song YJ, Wang YC, Li Y, Wang L, Zhang WD, Cheng J, Zhu Y, Zhang HY, Zhang Q, Niu HC, Zheng YW, Liang MY, Deng MQ, Shi H, Wang H, Zhang F, & Zhu ZB*. The whole transcriptome regulation as a function of mitochondrial polymorphisms and aging in Caenorhabditis elegans. Aging-US 2020; 12.

[27]Zhu ZB*, Li Y, Liang MY, Wang L, Wang L, Joshua R, Han CH*, & Zhang WD*. piRNAs Regulated by Mitochondria Variation Linked with Reproduction and Aging in Caenorhabditis elegans. Frontiers in Genetics 2020. doi.org/10.3389/fgene.2020.00190

[28]Li Y, Shan MZ, Li SH, Wang YC, Yang H, Chen Y, Gu B, & Zhu ZB*. Teasaponin suppresses Candida albicans filamentation by reducing the level of intracellular cAMP. Annals of Translational Medicine 2020. doi: 10.21037/atm.2020.01.124

[29]Li Y, Shan MZ, Yan MJ, Xu Y, Wang YC, Chen Y, Gu B, Li HC, & Zhu ZB*. The inhibition of trans-cinnamaldehyde on the virulence of Candida albicans via enhancing farnesol secretion with low potential for the development of resistance. Biochem Biophys Res Commun 2019; 515: 544-550.

[30]Li Y, Shan MZ, Yan M, Yao H, Wang YC, Gu B, Zhu ZB*, & Li HC*. Anticandidal Activity of Kalopanaxsaponin A: Effect on Proliferation, Cell Morphology, and Key Virulence Attributes of Candida albicans. Frontiers in Microbiology 2019; 10: 2844.

[31]Zhu ZB, Han XX, Wang YC, Liu W, Lu Y, Xu C, Wang XT, Hao L, Song YJ, Huang S, Rizak JD, Li Y, & Han C. Identification of Specific Nuclear Genetic Loci and Genes That Interact With the  Mitochondrial Genome and Contribute to Fecundity in Caenorhabditis elegans. Frontiers in Genetics 2019; 10: 28.

[32]Wang L, Wang M, Wise MJ, Liu Q, Yang T, Zhu ZB, Li C, Tan X, Tang D, & Wang W. Recent progress in the structure of glycogen serving as a durable energy reserve  in bacteria. World J Microbiol Biotechnol 2020; 36: 14.

[33]Liu QH^#, Zhu ZB^#, Wang MM^#, Wang Y, Zhang P, Wang HC, Liang MY, Li Y, Deng B, Tang DQ, Gilberti RG, Wang H, Liang MY, Li Y, & Wang L. Characterization of glycogen molecular structure in the worm Caenorhabditis elegans. CARBOHYDRATE POLYMERS 2020; 237: 116181.

[34]Zhu ZB, Han XX, Li Y, Han CH, Deng MQ, Zhang Y, ... & Hu FF. Identification of ROBO1/2 and SCEL as candidate genes in Kallmann syndrome with emerging bioinformatic analysis. 2019, Endocrine, 1-9.

[35][Zhu ZB, Lu Q, Zeng FF, Wang JJ, & Huang S. Compatibility between mitochondrial and nuclear genomes correlates with the quantitative trait of lifespan in Caenorhabditis elegans. Sci Rep 2015; 5: 1730

[36]Zhu ZB, Man X, Xia MY, Huang YM, Yuan DJ, & Huang S. Collective effects of SNPs on transgenerational inheritance in Caenorhabditis elegans and budding yeast. GENOMICS 2015; 106: 23-29.

[37]Zhu, ZB.., Lu, X., Yuan, D., Huang, S*. （2017）Close genetic relationships between a spousal pair with autism-affected children and high minor allele content in cases in autism-associated SNPs.GENOMICS 2017,109( 1), 9-15.

[38]Zhu, ZB., Lu, Q., Wang, J. & Huang, S*. (2015). Collective effects of common SNPs in foraging decisions in Caenorhabditis elegans and an integrative method of identification of candidate genes. Sci Rep, 2015, 5, 16904.

[39]Zhu, ZB., Yuan, D., Luo, D., Lu, X. & Huang, S*. (2015). Enrichment of Minor Alleles of Common SNPs and Improved Risk Prediction for Parkinson's Disease. PLoS One 10(7): e0133421.

[40]Yuan, D^#., Zhu, ZB^#., Tan, X., Liang, J., Zeng, C., Zhang, J., Chen, J., Ma, L., Dogan, A., Brockmann, G., Goldmann, O., Medina, E., Rice, A. D., Moyer, R. W., Man, X., Yi, K., Li, Y., Lu, Q., Huang, Y. & Huang, S*. (2014). Scoring the collective effects of SNPs: associations of minor alleles with complex traits in model organisms. Sci China Life Sci. 57：876-888.

[41]Zhou Z, Li Y, Ma Y, Zhang H, Deng Y, Zhu ZB*. Multi-biomarker is an early-stage predictor for progression of Coronavirus disease 2019 (COVID-19) infection. Int J Med Sci. 2021 May 27;18(13):2789-2798. doi: 10.7150/ijms.58742. PMID: 34220307; PMCID: PMC8241766.

[42]Xie X, Man X, Zhu ZB, Yuan D, Huang S. Tumor suppressor RIZ1 in obesity and the PI3K/AKT/mTOR pathway. Obesity (Silver Spring). 2016 Feb;24(2):389-97. doi: 10.1002/oby.21364. Epub 2015 Dec 26. PMID: 26709097.

[43]Hu Y, Hao M, Zhong C, An J, Zhang Y, Zhu ZB, Liu B, Cheng L. Discovery of an orally active antitumor agent that induces apoptosis and suppresses EMT through heat shock protein 90 inhibition. Invest New Drugs. 2021 Aug;39(4):1179-1188. doi: 10.1007/s10637-021-01083-9. Epub 2021 Mar 1. PMID: 33644823.

[44]Wang M, Liu Q, Kang X, Zhu ZB, Yang H, Xi X, Zhang X, Du Y, Guo M, Tang D, Wang L. Glycogen Metabolism Impairment via Single Gene Mutation in the glgBXCAP Operon Alters the Survival Rate of Escherichia coli Under Various Environmental Stresses. Front Microbiol. 2020 Sep 25;11:588099. doi: 10.3389/fmicb.2020.588099. PMID: 33101261; PMCID: PMC7546213.

[45]Wang L, Wang M, Wise MJ, Liu Q, Yang T, Zhu ZB, Li C, Tan X, Tang D, Wang W. Recent progress in the structure of glycogen serving as a durable energy reserve in bacteria. World J Microbiol Biotechnol. 2020 Jan 2;36(1):14. doi: 10.1007/s11274-019-2795-6. PMID: 31897771.

[46]Lei X, Yuan D, Zhu ZB, Huang S. Collective effects of common SNPs and risk prediction in lung cancer. Heredity (Edinb). 2018 Dec;121(6):537-547. doi: 10.1038/s41437-018-0063-4. Epub 2018 Mar 10. PMID: 29523840; PMCID: PMC6221896.

[47]He P, Lei X, Yuan D, Zhu ZB, Huang S. Accumulation of minor alleles and risk prediction in schizophrenia. Sci Rep. 2017 Sep 15;7(1):11661. doi: 10.1038/s41598-017-12104-0. PMID: 28916820; PMCID: PMC5601945.

[48]Guo JF, Zhang L, Li K, Mei JP, Xue J, Chen J, Tang X, Shen L, Jiang H, Chen C, Guo H, Wu XL, Sun SL, Xu Q, Sun QY, Chan P, Shang HF, Wang T, Zhao GH, Liu JY, Xie XF, Jiang YQ, Liu ZH, Zhao YW, Zhu ZB, Li JD, Hu ZM, Yan XX, Fang XD, Wang GH, Zhang FY, Xia K, Liu CY, Zhu XW, Yue ZY, Li SC, Cai HB, Zhang ZH, Duan RH, Tang BS. Coding mutations in NUS1 contribute to Parkinson's disease. Proc Natl Acad Sci U S A. 2018 Nov 6;115(45):11567-11572.

[49]Wang L, Wang M, Shi X, Yang J, Qian C, Liu Q, Zong L, Liu X, Zhu ZB, Tang D, Zhang X. Investigation into archaeal extremophilic lifestyles through comparative proteogenomic analysis. J Biomol Struct Dyn. 2021 Nov;39(18):7080-7092.

【中文教材】

朱作斌、张潇、王亮，系统生物学，东南大学出版社，ISBN:978-7-5766-0171-8,2022年，38万字。

【英文专著】

Wang L, Gu B，Zhang L，Zhu ZB, Recent Advances in Bacterial Biofilm Studies - Formation, Regulation, and Eradication in Human Infections. IntechOpen,  ISBN: 978-1-80356-709-9.

【科技奖励】

1. 中国中西医结合学会科技奖：精子发生及输送障碍性不育症中西医结合诊疗理论创新与临床应用，二等奖，2023年（排名第二）

2. 江苏省医学科技奖：精子发生及输送障碍关键技术创新与临床应用，三等奖，2023年（排名第一）

3. 江苏省高等学校科技成果奖：精、卵发生及输送障碍关键技术创新与应用，三等奖，2023年（排名第一）

4. 淮海科学技术奖：梗阻性无精子症外科治疗技术创新和推广应用，淮海科学技术奖，二等奖，2021年，2021-HJC-2-26-R02（排名第二）。

【发明专利】

1.      发明人：朱作斌、祝垚、李颖、施鹏、杜昕泽，2022年， 一种大肠杆菌BW25113菌株yjgW基因敲除突变株及其应用:CN202111396606.8. 中国

2.      发明人：朱作斌、杜昕泽、闵子桐、赵叙博、施朋、韩子冰、张璇，2022年，一种可检测线虫运动和行为并堆叠培养皿的培养箱:CN202210247051.中国

3.      发明人：杜昕泽、王元凡、朱作斌、张璇、曹轲鸣、顾云璐，2022年，一种培养基配置辅助置: CN202122825303.5. 中国

4.      发明人：朱作斌、张迎春、施朋、李颖、吴雅伦，2022年，大肠杆菌外膜囊泡的应用:CN202210299924.0.中国

5.      发明人：朱作斌、祝垚、施鹏、杜昕泽、李颖、臧泽升，2022年，一种大肠杆菌BW25113菌株ycgJ基因敲除突变株及其应用:CN202111371321.9. 中国

6.      发明人：金治宇、杜昕泽、朱作斌、施朋、王萧、孙晨译，2021年，一种基于深度学习与阈值分割的线虫识别方法:CN202110678930.2. 中国

7.      发明人：朱作斌、李颖、韩从辉，2021年，一种与非梗阻性无精子症诊断相关的血浆外泌体tsRNA标志物及其应用:CN202011023344.6.中国

8.      发明人：朱作斌、李颖、韩从辉，2021年，一种与原发性非梗阻性无精子症诊断相关的血浆外泌体miRNA标志物及其应用:CN202011023356.9. 中国

9.      发明人：朱作斌、李颖、韩从辉，2021年，一种与非梗阻性无精子症诊断相关的精浆外泌体tsRNA标志物及其应用:CN202011026301.3. 中国

10.   发明人：李颖、朱作斌、顾兵、单明珠、么焕开、李洪春、陈莹，2019年，刺楸皂苷A在制备抗白念珠菌药物中的应用及其药物制剂及白念珠菌引起的疾病药物制剂:CN201910676707.7. 中国

【软件著作权】

1.      著作权人：朱作斌、金治宇、张谦，基于深度学习算法应用于线虫、果蝇等小型模式动物生殖及衰老研究的高通量分析软件。

2.      著作权人：朱作斌、李颖、牛海晨，比较基因组学及DNA遗传多态性分析软件。

3.      著作权人：朱作斌、李颖、牛海晨，基于机器学习算法检查线虫社交行为软件。